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mgnify-jbrowse

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mgnify-jbrowse

## Usage

latest
npmnpm
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0.1.2
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Maintainers
1
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JBrowse Large Metageone Handler

Usage

Run npm install -force and then npm start to start a development instance

Run npm build which produces a build directory that can be deployed to a static web server

Generate Indexes

FTP URLs

FASTA File URL: https://www.ebi.ac.uk/metagenomics/api/v1/analyses/MGYA00516474/file/ERZ1049444_FASTA.fasta.gz GFF File URL: https://www.ebi.ac.uk/metagenomics/api/v1/analyses/MGYA00516474/file/ERZ1049444_FASTA_annotations.gff.bgz

Generating FASTA file indexes

Step 1: Decompress the FASTA File
$ gunzip -c ERZ1049444_FASTA.fasta.gz > ERZ1049444_FASTA.fasta
Step 2: Recompress as BGZF
$ bgzip ERZ1049444_FASTA.fasta
To confirm it's BGZF:
$ file ERZ1049444_FASTA.fasta.gz
Step 3: Create the FASTA Index
$ samtools faidx ERZ1049444_FASTA.fasta.gz

Generating GFF file indexes

Step 1: Decompress the BGZF file
$ bgzip -d -c ERZ1049444_FASTA_annotations.gff.bgz > ERZ1049444_FASTA_annotations.gff
Step 2: Recompress with Standard Gzip
$ gzip ERZ1049444_FASTA_annotations.gff
tabix index
$ tabix -p gff ERZ1049444_FASTA_annotations.gff.bgz
Step 3: Generate .ix and .ixx Index Files
$ jbrowse text-index --file ERZ1049444_FASTA_annotations.gff.bgz --exclude none --attributes interpro,pfam,eggnog 

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Package last updated on 24 Apr 2025

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