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bionode-bwa

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bionode-bwa - npm Package Compare versions

Comparing version 0.0.2 to 0.0.4

21

package.json
{
"name": "bionode-bwa",
"description": "A Node.js wrapper for the Burrow-Wheeler Aligner (BWA).",
"version": "0.0.2",
"version": "0.0.4",
"homepage": "http://github.com/bionode/bionode-bwa",

@@ -21,14 +21,17 @@ "repository": {

"devDependencies": {
"mocha": "~1.21.3",
"mocha-lcov-reporter": "0.0.1",
"should": "~4.0.4",
"async": "~0.9.0",
"coveralls": "~2.11.1",
"docco": "~0.6.3",
"istanbul": "~0.3.0",
"coveralls": "~2.11.1",
"async": "~0.9.0",
"request": "~2.39.0",
"tool-stream": "0.0.1",
"tap-spec": "^1.0.0",
"tape": "^3.0.0",
"testling": "^1.7.1",
"through2": "~0.5.1",
"docco": "~0.6.3"
"tool-stream": "0.0.1"
},
"keywords": [
"bio",
"biology",
"bionode",
"bioinformatics",

@@ -47,3 +50,3 @@ "aligner",

"preinstall": "git clone https://github.com/lh3/bwa.git; cd bwa; git checkout 0.7.10; make",
"test": "mocha --reporter spec; rm test/reference.*; rm test/reads.*; rm test/alignment.sam",
"test": "node test/bionode-bwa.js | tap-spec && rm test/reference.* && rm test/reads.* && rm test/alignment.sam",
"build-docs": "docco ./lib/bionode-bwa.js",

@@ -50,0 +53,0 @@ "coverage": "istanbul cover ./node_modules/.bin/_mocha --report lcovonly -- -R spec && rm -rf ./coverage; rm test/reference.*; rm test/reads.*; rm test/alignment.sam",

@@ -5,32 +5,28 @@ var bwa = require('../')

var request = require('request')
var should = require('should')
var tool = require('tool-stream')
var through = require('through2')
var async = require('async')
var test = require('tape')
require('mocha')
describe("Align reads to reference with BWA", function() {
this.timeout(6000000);
test("Align reads to reference with BWA", function (t) {
t.plan(2)
var referencePath = 'test/reference.fasta.gz'
var readsPath = 'test/reads.fastq.gz'
var alignmentPath = 'test/alignment.sam'
var referenceURL = 'http://ftp.ncbi.nlm.nih.gov/genbank/genomes/Eukaryotes/protozoa/Guillardia_theta/Guith1/Primary_Assembly/unplaced_scaffolds/FASTA/unplaced.scaf.fa.gz'
var readsURL = 'http://ftp.sra.ebi.ac.uk//vol1/fastq/SRR070/SRR070675/SRR070675.fastq.gz'
it("should take paths for reference, reads and aligment. Reference should be indexed first.", function(done) {
var referenceURL = 'http://ftp.ncbi.nlm.nih.gov/genbank/genomes/Eukaryotes/protozoa/Guillardia_theta/Guith1/Primary_Assembly/unplaced_scaffolds/FASTA/unplaced.scaf.fa.gz'
var readsURL = 'http://ftp.sra.ebi.ac.uk//vol1/fastq/SRR070/SRR070675/SRR070675.fastq.gz'
var downloads = [
download(referenceURL, referencePath),
download(readsURL, readsPath)
]
async.parallel(downloads, alignReadsToRef)
var downloads = [
download(referenceURL, referencePath),
download(readsURL, readsPath)
]
async.parallel(downloads, alignReadsToRef)
function alignReadsToRef() {
var mem = bwa() // default to mem
mem([referencePath, readsPath, alignmentPath])
.on('data', function(data) { checksum(data, '3dae171586e8f5fda2737795ff2e39df711701cb', done) })
}
})
function alignReadsToRef() {
var msg = "should take paths for reference, reads and aligment. Reference should be indexed first."
var mem = bwa() // default to mem
mem([referencePath, readsPath, alignmentPath])
.on('data', function(data) { checksum(data, '3dae171586e8f5fda2737795ff2e39df711701cb', msg, t) })
it("should align using a Stream that takes arrays of paths for reference and reads.", function(done) {
var msg = "should align using a Stream that takes arrays of paths for reference and reads."
var mem = bwa('mem -x pacbio')

@@ -40,4 +36,4 @@ var memStream = mem() // we will pass arguments with write

memStream.end()
memStream.on('data', function(data) { checksum(data, 'a2ac465ac0d9e0879a9f9a43108e36b682f2e018', done) })
})
memStream.on('data', function(data) { checksum(data, 'a2ac465ac0d9e0879a9f9a43108e36b682f2e018', msg, t) })
}
})

@@ -56,3 +52,3 @@

function checksum(data, hash, cb) {
function checksum(data, hash, msg, t) {
if (['index', 'aln'].indexOf(data.operation) === -1 && data.status === 'finished') {

@@ -64,6 +60,5 @@ var alignmentFile = fs.createReadStream(data.sam)

var sha1 = shasum.digest('hex');
sha1.should.eql(hash)
cb()
t.equal(sha1, hash, msg)
})
}
}

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