AutoLamella is a python package for automated cryo-lamella preparation with focused ion beam milling. It is based on openFIBSEM, and currently supports the TESCAN Automation SDK and ThermoFisher AutoScript. Support for other FIBSEM systems is planned.
Create a new virtual environment from the Anaconda Prompt terminal:
conda create -n fibsem python=3.9 pip
conda activate fibsem
pip install autolamella
Open the Anaconda Prompt terminal and run the following commands.
conda activate fibsem
autolamella_ui
To launch liftout related methods:
conda activate fibsem
autoliftout_ui
If you find this useful, please cite our work.
Genevieve Buckley, Gediminas Gervinskas, Cyntia Taveneau, Hariprasad Venugopal, James C. Whisstock, Alex de Marco, Automated cryo-lamella preparation for high-throughput in-situ structural biology, Journal of Structural Biology, Volume 210, Issue 2, 2020 https://doi.org/10.1016/j.jsb.2020.107488.
See CITATION for more details.
FAQs
Automatated ion beam milling for cryo-electron microscopy sample preparation.
We found that autolamella demonstrated a healthy version release cadence and project activity because the last version was released less than a year ago. It has 1 open source maintainer collaborating on the project.
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