hitips

HiTIPS

HiTIPS (High-Throughput Image Processing Software) is a comprehensive tool crafted for the analysis of high-throughput imaging datasets. Specifically designed for FISH (Fluorescence In Situ Hybridization) data, HiTIPS incorporates cutting-edge image processing and machine learning algorithms, delivering automated solutions for cell and nucleus segmentation, FISH signal identification, and quantification of signal attributes.

🌟 Key Features

• 🔍 Automated Segmentation: Efficiently segments cells and nuclei.
• 📍 FISH Signal Identification: Accurate localization and identification of FISH signals.
• 📊 Quantitative Analysis: Measures signal intensity and distribution.
• 🎨 Customizable Interface: Provides flexibility for customization and integrating plugins.
• 🚀 High-Throughput Support: Designed for processing large-scale imaging datasets.
• ⚙️ Extendable Algorithms: Incorporates new methodologies for enhancing current analysis routines.
• 🧩 Plugin Support: Supports the creation and integration of new analysis routines.

🔧 Hardware and Software Prerequisites

Hardware Requirements:

• CPU: Multi-core processor (e.g., Intel i7 or AMD Ryzen 7).
• RAM: Minimum 16GB (32GB recommended for large datasets).
• Storage: SSD with 500GB or more of available space.
• GPU: Optional but recommended, especially if using CUDA-enhanced functionalities.

Software Requirements:

• Operating System: 64-bit Linux distribution (e.g., Ubuntu, CentOS, Fedora).
• Python: Version 3.7 or newer.
• Package Manager: Latest version of Miniconda or Anaconda.

📥 Installation

Using Conda and Pip

1. Create a Conda Environment:

   conda create --name hitips_env python=3.8
   conda activate hitips_env
   
   

2. Install HiTIPS:

   pip install hitips
   
   

3. Launch HiTIPS:

   hitips
   

🚀 Usage

• Launch HiTIPS using the command python -m hitips.
• Introduce your high-throughput imaging dataset into the software.
• Navigate through the available analysis options and specify your desired tasks.
• Modify the analysis parameters fitting your requirements.
• Initiate the analysis process.
• Review and interpret the produced outcomes.
• Save or export the results as required.

🤝 Contributing

We warmly welcome contributions to HiTIPS! If you're keen on contributing, please adhere to the following guidelines:

• Fork and Branch: git checkout -b feature/your-feature-name
• Ensure that your changes align with the project's coding standards.
• Validate your modifications with appropriate tests.
• Commit your changes, ensuring your commit messages are descriptive.
• Push your updates to your fork.
• Submit a pull request on the primary HiTIPS repository detailing your changes.

📜 License

HiTIPS is distributed under the MIT License.

📞 Contact

For inquiries, feedback, or support, please don't hesitate to contact us at [e-mail].