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Automated cell patching extension for ACQ4
Install the package into your environment with e.g.
pip install acq4_autopatch
or
git clone https://github.com/sensapex/acq4-autopatch.git
conda develop acq4-autopatch
Customize the following to suit your hardware and add it to the modules:
section of your ACQ4 default.cfg
file:
Autopatch:
module: 'acq4_autopatch.module.AutopatchModule'
config:
imagingDevice: 'Camera'
patchDevices:
PatchPipette1: (0, 0) # bottom-left quad
PatchPipette2: (50*mm, 0) # bottom-right quad
PatchPipette3: (0, 50*mm) # top-left quad
PatchPipette4: (50*mm, 50*mm) # top-right quad
plateCenter: (0, 0, 0)
wellPositions: [(0, 0), (50*mm, 0), (0, 50*mm), (50*mm, 50*mm)]
patchStates:
cell detect:
maxAdvanceDistancePastTarget: 1*um
seal:
autoSealTimeout: 60
pressureMode: 'auto'
cell attached:
autoBreakInDelay: 5.0
clean:
approachHeight: 3*mm
cleanSequence: [(-35e3, 1.0), (65e3, 1.5)] * 5
rinseSequence: [(-35e3, 3.0), (65e3, 15.0)]
cameraChannels:
# Use different (exposure, trigger) DAQ lines for each pipette device
PatchPipette1: ('/Dev1/port0/line0', '/Dev1/port0/line1')
PatchPipette2: ('/Dev2/port0/line0', '/Dev2/port0/line1')
PatchPipette3: ('/Dev3/port0/line0', '/Dev3/port0/line1')
PatchPipette4: ('/Dev4/port0/line0', '/Dev4/port0/line1')
Briefly:
TODO create and then link to video explanation.
All software copyright (c) 2019-2020 Sensapex. All rights reserved. It is offered under multiple different licenses, depending on your needs:
FAQs
Automated cell patching extension for ACQ4
We found that acq4-autopatch demonstrated a healthy version release cadence and project activity because the last version was released less than a year ago. It has 2 open source maintainers collaborating on the project.
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